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Professor,
Biological Chemistry
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Director, Ph.D. Admissions Program,
ACCESS
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Member,
ACCESS Program: Dept. of Biological Chemistry
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ACCESS Affinity Group:
ACCESS Affinity - Cell Biology
Awards and Honors:
Lysosomes and Endocytosis Gordon Conference Chair, 2006
Lysosomes and Endocytosis Gordon Conference Vice-Chair, 2004
Pew Scholars Award, 1988-1992-01-01
Research Interest:
Protein transport within cells
Greg Payne’s lab investigates how proteins are transported between the commpartments of eukaryotic cells. In particular his lab focuses on two transport processes: 1) endocytosis, the process by which proteins and nutrients are taken into cells from their environment; 2) transport between two other compartments within the cell, the Golgi and endosomes, that is involved in sending proteins to the major degradation site in the cell, the lysosome. These pathways, which involve transport carriers called clathrin coated vesicles, are necessary for the normal functioning of most cells and defects in these pathways are associated with important diseases like cancer, heart disease, and Alzheimer’s disease.
The Payne group has made important discoveries that have helped understand how these transport processes work. For example, the lab discovered a role for the Golgi to endosome pathway in proper transport and functioning of a key enzyme involved in hormone and growth factor production. They also have identified and characterized several proteins that are critical for clathrin coated vesicle transport function in cells.
Dr. Payne’s current work is directed at using molecular genetics and imaging of living cells to understand how the proteins his group has identified contribute to the molecular mechanisms of clathrin coated vesicle formation during endocytosis and during transport between the Golgi and endosomes.
Biography:
Greg Payne is a cell biologist and geneticist studying mechanisms of intracellular protein transport. He joined the Department of Biological Chemistry in the UCLA School of Medicine in 1987, received tenure in 1994, became full professor in 2000, and was appointed departmental Vice Chair in 2004. Dr. Payne received his B.S. in Cell Biology with Honors in Drama from University of Michigan in 1977 and his Ph.D. in Biochemistry in the lab of Harold Varmus from University of California, San Francisco. He was a postdoctoral with Randy Schekman at University of California, Berkeley. He has served as curriculum coordinator for the joint Ph.D. admissions program, ACCESS, since its inception and became director in 2008. Dr. Payne research involves cell biological, biochemical, molecular and standard genetic and genomic approaches to understand vesicle mediated traffic in yeast.
Publications:
Duncan Mara C, Ho David G, Huang Jing, Jung Michael E, Payne Gregory S Composite synthetic lethal identification of membrane traffic inhibitors..
Proc. Natl. Acad. Sci. U.S.A..
2007; 104(15):
6235-40.
Piao Hai Lan, Machado Iara M P, Payne Gregory S NPFXD-mediated endocytosis is required for polarity and function of a yeast cell wall stress sensor..
Mol. Biol. Cell.
2007; 18(1):
57-65.
Mahadev Ravi K, Di Pietro Santiago M, Olson John M, Piao Hai Lan, Payne Gregory S, Overduin Michael Structure of Sla1p homology domain 1 and interaction with the NPFxD endocytic internalization motif..
EMBO J..
2007; 26(7):
1963-71.
Costaguta, G., Duncan, M.C., Fernandez, G.E., Huang, G.H. and Payne, G.S. Distinct Roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p.
Mol. Biol. Cell
2006; 17:
3907-3920.
Parsons, A.B., Lopez, A., Givoni, I.E., Williams, D.E., Gray, C.A., Porter, J., Chua, G., Sopko, R., Brost, R.L., Ho, C.-H., Wang, J., Ketela, T., Brenner, C., Brill, J.A., Esteban Fernandez , G., Lorenz, T.C., Payne, G.S., Ishihara, S., Ohya, Y., Andrews, B., Hughes, T.R., Frey, B.J., Graham, T.R., Andersen, R.J. and Boone, C. Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast..
Cell
2006; 126 (3):
611-625.
Fernandez, G. Esteban and Payne, G.S. Laa1p, a conserved AP-1 accessory protein important for AP-1 localization in yeast. .
Mol. Biol. Cell
2006; 17:
3304-3317.
Duncan, M.C. and Payne, G.S. Protein Choreography.
Nature
2005; 438 571-573:
571-573.
Duncan, MC Costaguta, G Payne, GS Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif..
Nature cell biology. .
2003; 5(1):
77-81.
Howard, J. P., Hutton, J.L., Olson, J.M. and Payne, G.S. Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis..
J. Cell Biol.
2002; 157:
315-326.
Yeung, BG Payne, GS Clathrin interactions with C-terminal regions of the yeast AP-1 beta and gamma subunits are important for AP-1 association with clathrin coats..
Traffic (Copenhagen, Denmark) .
2001; 2(8):
565-76.
Dell'Angelica, EC Payne, GS Intracellular cycling of lysosomal enzyme receptors: cytoplasmic tails' tales..
Cell. .
2001; 106(4):
395-8.
Bensen, ES Yeung, BG Payne, GS Ric1p and the Ypt6p GTPase function in a common pathway required for localization of trans-Golgi network membrane proteins..
Molecular biology of the cell. .
2001; 12(1):
13-26.
Costaguta, G Stefan, CJ Bensen, ES Emr, SD Payne, GS Yeast Gga coat proteins function with clathrin in Golgi to endosome transport..
Molecular biology of the cell. .
2001; 12(6):
1885-96.
Pishvaee, B Costaguta, G Yeung, BG Ryazantsev, S Greener, T Greene, LE Eisenberg, E McCaffery, JM Payne, GS A yeast DNA J protein required for uncoating of clathrin-coated vesicles in vivo..
Nature cell biology. .
2000; 2(12):
958-63.
Bensen, E., Costaguta, G. and Payne, G.S. Synthetic interactions with temperature-sensitive clathrin in Saccharomyces cerevisiae: roles for dynamin-related Vps1p and synaptojanin-like Inp53p in clathrin-dependent TGN membrane protein localization..
Genetics
2000; 154:
83-97.
Chu, DS Pishvaee, B Payne, GS A modulatory role for clathrin light chain phosphorylation in Golgi membrane protein localization during vegetative growth and during the mating response of Saccharomyces cerevisiae..
Molecular biology of the cell. .
1999; 10(3):
713-26.
Vowels, JJ Payne, GS A dileucine-like sorting signal directs transport into an AP-3-dependent, clathrin-independent pathway to the yeast vacuole..
The EMBO journal. .
1998; 17(9):
2482-93.
Pishvaee, B Munn, A Payne, GS A novel structural model for regulation of clathrin function..
The EMBO journal. .
1997; 16(9):
2227-39.
Tan, PK Howard, JP Payne, GS The sequence NPFXD defines a new class of endocytosis signal in Saccharomyces cerevisiae..
The Journal of cell biology. .
1996; 135(6 Pt 2):
1789-800.
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