Department / Division Affiliations
Assistant Professor,
Biological Chemistry
Awards and Honors:
UCLA School of Medicine, Deans Office, 1st John H. Walsh Research Prize, UCLA, 2009
2008 CIRM CIRM Young Investigator Award
2007 National Institute of Health NIH Director's New Innovator Award
2007 Kimmel Foundation Scholar
2007 V Foundation Scholar
2001 Junior Scientist Award of the States Berlin and Brandenburg, Germany
2000 Byk-Gulden Graduate Thesis Award of the German Society of Biochemistry
International Society for Stem Cell Research, Board of Directors
UCLA ACCESS PhD Program Admission Committee
UCLA MDPhD Program (MSTP) Admission Committee
UCLA, MBIDP Ph.D. Committee
2004 Special Fellow of the Leukemia and Lymphoma Society
2001 Life Sciences Research Foundation Postdoctoral Fellowship
Research Interest:
Epigenetic regulation of stem cells and cell fate changes during differentiation and reprogramming
Born in Germany, Kathrin Plath earned her doctorate degree in cell biology from Harvard Medical School and the Humboldt University Berlin, Germany, and did her post-doctoral training at the University of California, San Francisco, where she worked on the X-inactivation process in female mammalian cells. In 2003, Plath moved to the Whitehead Institute at MIT to gain expertise in stem cell biology. She joined UCLAs Biological Chemistry Department in March 2006. The Plath lab focuses on epigenetic changes that occur during differentiation of pluripotent cells or during reprogramming to pluripotency, and is interested in understanding the mechanisms that maintain the pluripotent state. To address these questions, we are using a combination of mammalian tissue culture systems, fluorescence microscopy, biochemistry and mass spectrometry, RNAi and overexpression approaches, next generation sequencing to reveal RNA expression, DNA methylation, histone modification and transcription factor binding patterns, and transgenic/knockout mouse models.
Publications:
Diaz Perez Silvia V, Kim Rachel, Li Ziwei, Marquez Victor E, Patel Sanjeet, Plath Kathrin, Clark Amander T Derivation of new human embryonic stem cell lines reveals rapid
epigenetic progression in vitro that can be prevented by chemical
modification of chromatin..
Human molecular genetics.
2012; 21(4):
751-64.
Minkovsky Alissa, Patel Sanjeet, Plath Kathrin Concise review: Pluripotency and the transcriptional inactivation of
the female Mammalian X chromosome..
Stem cells (Dayton, Ohio).
2012; 30(1):
48-54.
Cooper Aaron R, Patel Sanjeet, Senadheera Shantha, Plath Kathrin, Kohn Donald B, Hollis Roger P Highly efficient large-scale lentiviral vector concentration by tandem tangential flow filtration..
Journal of Virological Methods.
2011; 177(1):
1-9.
Sridharan Rupa, Plath Kathrin Small RNAs loom large during reprogramming..
Cell Stem Cell.
2011; 8(6):
599-601.
Ho Ritchie, Chronis Constantinos, Plath Kathrin Mechanistic insights into reprogramming to induced pluripotency..
Journal of Cellular Physiology.
2011; 226(4):
868-78.
Plath Kathrin, Lowry William E Progress in understanding reprogramming to the induced pluripotent state..
Nature Reviews Genetics.
2011; 12(4):
253-65.
Papp Bernadett, Plath Kathrin Reprogramming to pluripotency: stepwise resetting of the epigenetic landscape..
Cell Research.
2011; 21(3):
486-501.
Mason Mike J, Plath Kathrin, Zhou Qing Identification of context-dependent motifs by contrasting ChIP binding data..
Bioinformatics (Oxford, England).
2010; 26(22):
2826-32.
Mayshar Yoav, Ben-David Uri, Lavon Neta, Biancotti Juan-Carlos, Yakir Benjamin, Clark Amander T, Plath Kathrin, Lowry William E, Benvenisty Nissim Identification and classification of chromosomal aberrations in human induced pluripotent stem cells..
Cell Stem Cell.
2010; 7(4):
521-31.
Tchieu Jason, Kuoy Edward, Chin Mark H, Trinh Hung, Patterson Michaela, Sherman Sean P, Aimiuwu Otaren, Lindgren Anne, Hakimian Shahrad, Zack Jerome A, Clark Amander T, Pyle April D, Lowry William E, Plath Kathrin Female human iPSCs retain an inactive X chromosome..
Cell Stem Cell.
2010; 7(3):
329-42.
Chin Mark H, Pellegrini Matteo, Plath Kathrin, Lowry William E Molecular analyses of human induced pluripotent stem cells and embryonic stem cells..
Cell Stem Cell.
2010; 7(2):
263-9.
Hiratani Ichiro, Ryba Tyrone, Itoh Mari, Rathjen Joy, Kulik Michael, Papp Bernadett, Fussner Eden, Bazett-Jones David P, Plath Kathrin, Dalton Stephen, Rathjen Peter D, Gilbert David M Genome-wide dynamics of replication timing revealed by in vitro models of mouse embryogenesis..
Genome Research.
2010; 20(2):
155-69.
Xu Jian, Watts Jason A, Pope Scott D, Gadue Paul, Kamps Mark, Plath Kathrin, Zaret Kenneth S, Smale Stephen T Transcriptional competence and the active marking of tissue-specific enhancers by defined transcription factors in embryonic and induced pluripotent stem cells..
Genes & Development.
2009; 23(24):
2824-38.
Gaspar-Maia Alexandre, Alajem Adi, Polesso Fanny, Sridharan Rupa, Mason Mike J, Heidersbach Amy, Ramalho-Santos João, McManus Michael T, Plath Kathrin, Meshorer Eran, Ramalho-Santos Miguel. Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
Nature.
2009; 460(7257):
863-8.
Mason Mike J, Fan Guoping, Plath Kathrin, Zhou Qing, Horvath Steve. Signed weighted gene co-expression network analysis of transcriptional regulation in murine embryonic stem cells.
BMC Genomics.
2009; 10(7257):
327.
Chin Mark H, Mason Mike J, Xie Wei, Volinia Stefano, Singer Mike, Peterson Cory, Ambartsumyan Gayane, Aimiuwu Otaren, Richter Laura, Zhang Jin, Khvorostov Ivan, Ott Vanessa, Grunstein Michael, Lavon Neta, Benvenisty Nissim, Croce Carlo M, Clark Amander T, Baxter Tim, Pyle April D, Teitell Mike A, Pelegrini Matteo, Plath Kathrin, Lowry William E. Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures.
Cell Stem Cell.
2009; 5(1):
111-23.
Park Tae Sub, Galic Zoran, Conway Anne E, Lindgren Anne, van Handel Benjamin J, Magnusson Mattias, Richter Laura, Teitell Michael A, Mikkola Hanna K A, Lowry William E, Plath Kathrin, Clark Amander T. Derivation of primordial germ cells from human embryonic and induced pluripotent stem cells is significantly improved by coculture with human fetal gonadal cells.
Stem Cells.
2009; 27(4):
783-95.
Karumbayaram Saravanan, Novitch Bennett G, Patterson Michaela, Umbach Joy A, Richter Laura, Lindgren Anne, Conway Anne E, Clark Amander T, Goldman Steve A, Plath Kathrin, Wiedau-Pazos Martina, Kornblum Harley I, Lowry William E. Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
Stem Cells.
2009; 27(4):
806-11.
Daley George Q, Lensch M William, Jaenisch Rudolf, Meissner Alex, Plath Kathrin, Yamanaka Shinya. Broader implications of defining standards for the pluripotency of iPSCs.
Cell Stem Cell.
2009; 4(3):
200-1.
Hochedlinger Konrad, Plath Kathrin. Epigenetic reprogramming and induced pluripotency.
Development.
2009; 136(4):
509-23.
Xie Wei, Song Chunying, Young Nicolas L, Sperling Adam S, Xu Feng, Sridharan Rupa, Conway Anne E, Garcia Benjamin A, Plath Kathrin, Clark Amander T, Grunstein Michael. Histone h3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells.
Molecular Cell.
2009; 33(4):
417-27.
Saxe Jonathan P, Tomilin Alexey, Schöler Hans R, Plath Kathrin, Huang Jing. Post-translational regulation of Oct4 transcriptional activity.
PloS One.
2009; 4(2):
e4467.
Sridharan Rupa, Tchieu Jason, Mason Mike J, Yachechko Robin, Kuoy Edward, Horvath Steve, Zhou Qing, Plath Kathrin. Role of the murine reprogramming factors in the induction of pluripotency.
CELL.
2009; 136(2):
364-77.
Lowry William E and Plath Kathrin. The many ways to make an iPS cell.
Nature Biotechnology.
2008; 26(11):
1246-8.
Sridharan Rupa and Plath Kathrin. Illuminating the black box of reprogramming.
Cell Stem Cell.
2008; 2(4):
295-7.
Schenke-Layland Katja, Rhodes Katrin E, Angelis Ekaterini, Butylkova Yekaterina, Heydarkhan-Hagvall Sepideh, Gekas Christos, Zhang Rui, Goldhaber Joshua I, Mikkola Hanna K, Plath Kathrin, MacLellan W Robb Reprogrammed mouse fibroblasts differentiate into cells of the cardiovascular and hematopoietic lineages..
Stem Cells.
2008; 26(6):
1537-46.
Lowry W E, Richter L, Yachechko R, Pyle A D, Tchieu J, Sridharan R, Clark A T, Plath K Generation of human induced pluripotent stem cells from dermal fibroblasts..
Proc. Natl. Acad. Sci. U.S.A..
2008; 105(8):
2883-8.
Maherali, N.#, Sridharan, R.#, Xie, W., Utikal, J., Eminli, S., Arnold, K., Stadtfeld, M., Yachechko, R., Tchieu. J., Jaenisch, R., Plath, K.*, and Hochedlinger, K*. Directly Reprogrammed Fibroblasts Show Global Epigenetic Remodeling and Widespread Tissue Contribution.
Cell Stem Cell
2007; 1(1):
55-70 #equal contribution; * co-corresponding authors.
Nusinow Dmitri A, Sharp Judith A, Morris Alana, Salas Sonia, Plath Kathrin, Panning Barbara The histone domain of macroH2A1 contains several dispersed elements that are each sufficient to direct enrichment on the inactive X chromosome..
J. Mol. Biol..
2007; 371(1):
11-8.
Bernstein BE, Mikkelsen TS, Xie X, Kamal M, Huebert DJ, Cuff J, Fry B, Meissner A, Wernig M, Plath K, Jaenisch R, Wagschal A, Feil R, Schreiber SL, Lander ES A bivalent chromatin structure marks key developmental genes in embryonic stem cells.
Cell.
2006; 125(2):
315-26.
Boyer LA*, Plath K*, Zeitlinger J, Brambrink T,
Medeiros LA, Lee TI, Levine SS, Wernig M, Tajonar A,
Ray MK, Bell GW, Otte AP, Vidal M, Gifford DK, Young RA, Jaenisch R Polycomb complexes repress developmental regulators in murine embryonic stem cells.
Nature.
2006; 441:
349-353 * both authors contributed equally.
Chu F, Nusinow DA, Chalkley RJ, Plath K, Panning B, Burlingame AL Mapping post-translational modifications of the histone variant MacroH2A1 using tandem mass spectrometry.
Mol Cell Proteomics.
2006; 5(1):
194-203.
Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells.
Genesis.
2006; 44(1):
23-8.
de la Cruz CC, Fang J, Plath K, Worringer KA, Nusinow DA, Zhang Y, Panning B Developmental regulation of Suz 12 localization.
Chromosoma.
2005; 114(3):
183-92.
Plath K, Talbot D, Hamer KM, Otte AP, Yang TP, Jaenisch R, Panning B Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome.
J Cell Biol.
2004; 167(6):
1025-35.
Plath K, Wilkinson BM, Stirling CJ, Rapoport TA Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum.
Mol Biol Cell.
2004; 15(1):
1-10.
Plath K*, Fang J*, Mlynarczyk-Evans SK, Cao R, Worringer KA, Wang H, de la Cruz CC, Otte AP, Panning B, Zhang Y Role of histone H3 lysine 27 methylation in X inactivation.
Science.
2003; 300(5616):
131-5 * both authors contributed equally.
Plath K, Mlynarczyk-Evans S, Nusinow DA, Panning B Xist RNA and the mechanism of X chromosome inactivation.
Annu Rev Genet.
2002; 36:
233-78.
Plath K, Rapoport TA Spontaneous release of cytosolic proteins from posttranslational substrates before their transport into the endoplasmic reticulum.
J Cell Biol.
2000; 151(1):
167-78.
Menetret JF, Neuhof A, Morgan DG, Plath K, Radermacher M, Rapoport TA, Akey CW The structure of ribosome-channel complexes engaged in protein translocation.
Mol Cell.
2000; 6(5):
1219-32.
Matlack KE, Misselwitz B, Plath K, Rapoport TA BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membrane.
Cell.
1999; 97(5):
553-64.
Rapoport TA, Matlack KE, Plath K, Misselwitz B, Staeck O Posttranslational protein translocation across the membrane of the endoplasmic reticulum.
Biol Chem.
1999; 380(10):
1143-50.
Plath K, Mothes W, Wilkinson BM, Stirling CJ, Rapoport TA Signal sequence recognition in posttranslational protein transport across the yeast ER membrane.
Cell.
1998; 94(6):
795-807.
Matlack KE, Plath K, Misselwitz B, Rapoport TA Protein transport by purified yeast Sec complex and Kar2p without membranes.
Science.
1997; 277(5328):
938-41.
Hanein D, Matlack KE, Jungnickel B, Plath K, Kalies KU, Miller KR, Rapoport TA, Akey CW Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.
Cell.
1996; 87(4):
721-32.
Finke K*, Plath K*, Panzner S, Prehn S, Rapoport TA, Hartmann E, Sommer T A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae.
Embo J.
1996; 15(7):
1482-94 * both authors contributed equally.
Engel K, Schultz H, Martin F, Kotlyarov A, Plath K, Hahn M, Heinemann U, Gaestel M Constitutive activation of mitogen-activated protein kinase-activated protein kinase 2 by mutation of phosphorylation sites and an A-helix motif.
J Biol Chem.
1995; 270(45):
27213-21.
Plath K, Engel K, Schwedersky G, Gaestel M Characterization of the proline-rich region of mouse MAPKAP kinase 2: influence on catalytic properties and binding to the c-abl SH3 domain in vitro.
Biochem Biophys Res Commun.
1994; 203(2):
1188-94.
Engel K, Plath K, Gaestel M The MAP kinase-activated protein kinase 2 contains a proline-rich SH3-binding domain.
FEBS Lett.
1993; 336(1):
143-7.
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